Article Essay Example | Topics and Well Written Essays - 1500 words - 1

Article - Essay Example (Krawczyk, M.et.al, 2008, p. 136). The study concludes that the CC-genotype, BTH and genotype correlate perfectly, and the genetic test provides an unambiguous result. In both positive individual with a negative genetic test there is good reason to suspect secondary causes of lactase deficiency. (Krawczyk, M.et.al, 2008, p.138). In the study 58 consecutives patients are chosen (25 males, 33 females; median age 41 years, range 18 – 82 years) recruited prospectively between April 2005 and July 2007. (Krawczyk, M.et.al, 2008, p.137). These patients were referred by the department with non-specific gastrointestinal symptoms consistent with lactose intolerance (i.e., bloating, abdominal pain, diarrhea).All participants signed an informed consent form, and the study was conducted according to a study design approved by the local ethical committee. (Krawczyk, M.et.al, 2008, p.137). The method in genetic test includes the use of a Peripheral venous blood samples for DNA testing were obtained from all patients. DNA was isolated using the DNeasy Blood & Tissue kit (Qiagen, Hilden, Germany). The genotyping procedure consisted of polymerase chain reaction (PCR) amplification and SNP detection of the -13,910C>T variant using SNaPshot minisequencing (Applera, Norwalk,CT). (Krawczyk, M.et.al, 2008, p.136). For Hydrogen breathe test (BTH), it was performed after at least 12 hours overnight fasting. All patients were obliged to restrain from cigarette smoking before the test. Additionally, individuals who underwent colonoscopy or were taking any antibiotics in the fortnight before the test were excluded from the study. The test was performed after ingestion of 50 g of lactose diluted in 300 ml of water. The amount of exhaled hydrogen was measured in parts per million (ppm) before lactose ingestion (baseline), every 10 minutes during the first hour and every 20 minutes